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2 edition of effects of T-antigen and transcription factor binding sites on simian virus 40 DNA replication found in the catalog.

effects of T-antigen and transcription factor binding sites on simian virus 40 DNA replication

Paula Jo Wilderman

effects of T-antigen and transcription factor binding sites on simian virus 40 DNA replication

by Paula Jo Wilderman

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Published .
Written in English

    Subjects:
  • SV40 (Virus),
  • DNA replication,
  • Antigens

  • Edition Notes

    Statementby Paula Jo Wilderman
    The Physical Object
    Paginationxii, 170 leaves :
    Number of Pages170
    ID Numbers
    Open LibraryOL15253797M

    ysis of tail DNA using the kilobase SV40 large T antigen DNA fragment from the original construct as the probe. The integrity of the construct was analyzed by Bam\\\IXba\ restriction enzyme digestion of tail DNA followed by Southern blot analysis. The abundance of the DNA was assessed by staining the agarose gel prior to transfer to nylon. Joo, W. S. et al. Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin. J. Virol. 71, – ().

    Simian virus 40 (SV40) large T antigen is a potent transcriptional activator of both viral and cellular theSV40latepromoter,aspecific upstreamelementnecessaryforT-antigentranscriptional activation is the binding site fortranscription-enhancing factor 1 .   Proc Naa Acad Sci USA , x.x, Pulse-chase experiments show that a highly unwound form of DNA is a preferred substrate for replication initiation in vitro. TStmiMOTO T, FAmMAN MP, STnLMAN B: Simian virus 40 DNA replication in vttr~ Identification of multiple stages of ini- tiation. Mo/ Ce//BR)/ ,

    Simian virus 40 Subject Areas on Research A gamma-ray-resistant derivative of an ataxia telangiectasia cell line obtained following DNA-mediated gene transfer.   The binding of pRb to large T-antigen is not required for the replication of SV40 as the ability of T-antigen to mediate transcriptional activation of viral genes and viral DNA replication occurs.


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Effects of T-antigen and transcription factor binding sites on simian virus 40 DNA replication by Paula Jo Wilderman Download PDF EPUB FB2

Abstract. Simian virus 40 (SV40) large T antigen is a potent transcriptional activator of both viral and cellular promoters. Within the SV40 late promoter, a specific upstream element necessary for T-antigen transcriptional activation is the binding site for transcription-enhancing factor 1 (TEF-1).Cited by: The origins of DNA replication (ori) in simian virus 40 (SV40) and polyomavirus (Py) contain an auxiliary component (aux-2) composed of multiple transcription factor binding sites.

To determine whether this component stimulated replication by binding specific transcription factors, aux-2 was replaced by synthetic oligonucleotides that bound a Cited by: The late promoter of simian virus 40 is transcriptionally activated, in trans, by large T antigen, the primary viral early gene product.

Although large T antigen is a well-characterized DNA-binding protein, a variety of data suggest that its trans-activation Cited by: The origins of DNA replication (ori) in simian virus 40 (SV40) and polyomavirus (Py) contain an auxiliary component (aux-2) composed of multiple transcription factor binding sites.

We have characterized a series of simian virus 40 (SV40) mutants with defined deletions or base-pair substitutions in the viral regulatory region near Cited by: Simian virus 40 (SV40) large tumor (T) antigen is a highly complex protein which performs a wide variety of functions during the viral life cycle (reviewed in references 28 and 29).Early after infection, T antigen begins to accumulate and alters cell growth regulation, in part by direct binding to the tumor suppressor proteins Rb, p, p, and p53 (reviewed in references 13 and 63) and to.

Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro Article (PDF Available) in Journal of Virology 73(2) The expression of the Simian virus large-T antigen gene (SVTag) in transgenic mice can result in the immortalization and developmental arrest of desired cell types.

4, 5 Mellon's group, for example, has generated cells locked at different stages of pituitary development using this approach. 6, 7 They used a short version of the LH-β.

Programmed factor binding to simian virus 40 GC-box replication and transcription control sequences. The program was interrupted by the DNA replication inhibitor aphidicolin or by inactivation of the viral replication factor simian virus 40 T antigen, suggesting a link between viral DNA replication and new factor binding.

the data point. Gallo GJ, Gilinger G, Alwine JC. Simian virus 40 T antigen alters the binding characteristics of specific simian DNA-binding factors. Mol Cell Biol. Apr; 8 (4)– [Europe PMC free article] [Google Scholar] Gilman MZ, Wilson RN, Weinberg RA.

Multiple protein-binding sites in the 5'-flanking region regulate c-fos expression. SUMMARY Simian virus 40 (SV40) is a small DNA tumor virus that has been extensively characterized due to its relatively simple genetic organization and the ease with which its genome is manipulated.

The large and small tumor antigens (T antigens) are the major regulatory proteins encoded by SV Large T antigen is responsible for both viral and cellular transcriptional regulation, virion. DiMaio D, Nathans D. Regulatory mutants of simian virus Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.

J Mol Biol. Apr 15; (3)– [Google Scholar] Dixon RA, Nathans D. Purification of simian virus 40 large T antigen by immunoaffinity chromatography. J Virol. INTRODUCTION.

Simian virus 40 (SV40), a member of the Polyomaviridae family, has a rich history of discovery. Its use as a model system has led to fundamental insights into the molecular processes of genome replication, gene expression, posttranscriptional processing, and cell cycle regulation.

DiMaio D, Nathans D. Regulatory mutants of simian virus Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. J Mol Biol. Apr 15; (3)– Myers RM, Tjian R. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.

Phosphorylation of simian virus 40 (SV40) T antigen on threonine activates viral DNA replication in vivo and in vitro. We have manipulated the modification of T-antigen residue both. The alternative splicing of the T-antigen gene of simian virus 40 (SV40) is regulated by the presence of splicing factor 2 (SF2), which is a(n) _____ protein that contains two domains, an RNA-binding region and another domain with alternating serine and arginine amino acids.

Simian virus 40 large tumor antigen (SV40 T antigen) untwists DNA at the SV40 replication origin. In the presence of ATP, T antigen shifted the average linking number of an SV40 origin-containing plasmid topoisomer distribution.

The loss of up to two helical turns was detected. To directly test the effect of pRb inactivation on antiestrogen sensitivity, we have expressed the simian virus 40 (SV) or polyoma virus (Py) large T antigens (LTs) in MCF-7 cells. SV40 T antigen interactions with ssDNA and replication protein A: a regulatory role of T antigen monomers in lagging strand DNA replication USP10 deubiquitylates the histone variant H2A.Z and both are required for androgen receptor-mediated gene activation.

DNA tumor viruses such as simian virus 40 (SV40) express dominant acting oncoproteins that exert their effects by associating with key cellular targets and altering the signaling pathways they govern. The Simian Virus 40 (SV40) large T antigen (T) is required for the initiation of viral replication, the autoregulation of early gene expression, and the activation of late gene expression in.Initiates viral DNA replication and unwinding via interactions with the viral origin of replication.

Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late .Essential nucleotide contacts between the SV40 large T (tumor) antigen and binding sites I and II on the SV40 genome have been inferred from in vitro methylation- and ethylation-interference experiments.

Each site contains two clusters of guanine residues that reduce the specific binding of T antigen when modified.

Methylation at any one of nine guanines within site I or any one of five.